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The biological functions of the human body are complex and over the years, scientists and researchers have carried out research to discover the human anatomy. The major building block of the human body is the cell. They are the structural compositions of the body and are mostly referred to as the smallest basic units of life. In order to obtain a more comprehensive understanding of the functioning of these units, live cell microscopy is applied.
This imaging method has been accessible for many years now and has been extended to many different fields. It involves viewing of these structural units under a time-lapse microscope in order to study their cellular structure. Since its pioneering, several methods of such imaging have been developed which allows these units to be studied in great detail. This enhances the knowledge on functions of cellular structures and tissues.
When you are undertaking the study, it is important to have good maintenance of the specimen. This means that if you expose them to certain environments that may stress-inducing, they will not give the appropriate imagery and give wrong information. Stressful conditions or environments are believed to cause an alteration in the biological and physiological processes of these structures.
Live-cell fluorescent imaging is able to assist in the creation of high-quality images by using fluorescent proteins to stain them. This allows the images obtained to be multicolored. These techniques are aimed at reducing the reflected light commonly known as the incident light by using fluorescent light. This reduces the photo-toxicity or photo-bleaching as a result of reduced incident light and lack of ultraviolet light as well.
So as to have a clear live-cell imagery, you have to make sure the light you are using is a fluorescent one and minimize any cases of incident light which may contain phototoxic characteristics. Actually, most structures have been functioning in light exposure it is therefore important to reduce this light when making imageries applications. The microscope should be the object to collect light but not exposing the specimen to the light.
This study involves maintaining the balance between obtaining images that are of high quality while also maintaining the cellular health of these structural units. These units are prone to photodamage, especially when there is a presence of fluorophores. Even in the absence of fluorophores, mammalian cellular structures are still sensitive to ultraviolet light.
When choosing an optimal imaging system, there are several factors to be considered. These factors include the sensitivity of the detector, the viability of specimen and the required speed for image acquisition. It should make maximum use of light and also use few optical elements in the path of light. Many different types of fluorescent proteins of different colors are available to be used as fluorescent tags.
The benefits from such experiments include examples like monitoring molecular interaction, viral replication, and testing temperature dependency of drug-induced events. It is however disadvantageous since the cellular structures are damaged at the end of an experiment.
This imaging method has been accessible for many years now and has been extended to many different fields. It involves viewing of these structural units under a time-lapse microscope in order to study their cellular structure. Since its pioneering, several methods of such imaging have been developed which allows these units to be studied in great detail. This enhances the knowledge on functions of cellular structures and tissues.
When you are undertaking the study, it is important to have good maintenance of the specimen. This means that if you expose them to certain environments that may stress-inducing, they will not give the appropriate imagery and give wrong information. Stressful conditions or environments are believed to cause an alteration in the biological and physiological processes of these structures.
Live-cell fluorescent imaging is able to assist in the creation of high-quality images by using fluorescent proteins to stain them. This allows the images obtained to be multicolored. These techniques are aimed at reducing the reflected light commonly known as the incident light by using fluorescent light. This reduces the photo-toxicity or photo-bleaching as a result of reduced incident light and lack of ultraviolet light as well.
So as to have a clear live-cell imagery, you have to make sure the light you are using is a fluorescent one and minimize any cases of incident light which may contain phototoxic characteristics. Actually, most structures have been functioning in light exposure it is therefore important to reduce this light when making imageries applications. The microscope should be the object to collect light but not exposing the specimen to the light.
This study involves maintaining the balance between obtaining images that are of high quality while also maintaining the cellular health of these structural units. These units are prone to photodamage, especially when there is a presence of fluorophores. Even in the absence of fluorophores, mammalian cellular structures are still sensitive to ultraviolet light.
When choosing an optimal imaging system, there are several factors to be considered. These factors include the sensitivity of the detector, the viability of specimen and the required speed for image acquisition. It should make maximum use of light and also use few optical elements in the path of light. Many different types of fluorescent proteins of different colors are available to be used as fluorescent tags.
The benefits from such experiments include examples like monitoring molecular interaction, viral replication, and testing temperature dependency of drug-induced events. It is however disadvantageous since the cellular structures are damaged at the end of an experiment.
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